The STING phase-separator suppresses innate immune signalling.
Nat Cell Biol
; 23(4): 330-340, 2021 04.
Article
em En
| MEDLINE
| ID: mdl-33833429
ABSTRACT
Biomolecular condensates (biocondensates) formed via liquid-liquid phase-separation of soluble proteins have been studied extensively. However, neither the phase-separation of endoplasmic reticulum (ER) transmembrane protein nor a biocondensate with organized membranous structures has been reported. Here, we have discovered a spherical ER membranous biocondensate with puzzle-like structures caused by condensation of the ER-resident stimulator of interferon genes (STING) in DNA virus-infected or 2'3'-cGAMP (cyclic GMP-AMP)-treated cells, which required STING transmembrane domains, an intrinsically disordered region (IDR) and a dimerization domain. Intracellular 2'3'-cGAMP concentrations determined STING translocation or condensation. STING biocondensates constrained STING and TBK1 (TANK binding protein 1) to prevent innate immunity from overactivation, presumably acting like a 'STING-TBK1-cGAMP sponge'. Cells expressing STING-E336G/E337G showed notably enhanced innate immune responses due to impaired STING condensation after viral infection at later stages. Microtubule inhibitors impeded the STING condensate gel-like transition and augmented type I-interferon production in DNA virus-infected cells. This membranous biocondensate was therefore named the STING phase-separator.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retículo Endoplasmático
/
Extração Líquido-Líquido
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Imunidade Inata
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Proteínas de Membrana
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article