Functional diversity of three tandem C-terminal carbohydrate-binding modules of a ß-mannanase.

Møller, Marie Sofie; El Bouaballati, Souad; Henrissat, Bernard; Svensson, Birte

Møller, Marie Sofie; El Bouaballati, Souad; Henrissat, Bernard; Svensson, Birte.

Afiliação

Møller MS; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark. Electronic address: msmo@dtu.dk.
El Bouaballati S; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.
Henrissat B; Architecture et Fonction des Macromolécules Biologiques, CNRS, Aix-Marseille Université, Marseille, France; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Svensson B; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.

J Biol Chem ; 296: 100638, 2021.

Article em En | MEDLINE | ID: mdl-33838183

Assuntos

Celulose/metabolismo; Gammaproteobacteria/enzimologia; Mananas/metabolismo; beta-Manosidase/metabolismo; Sequência de Aminoácidos; Domínio Catalítico; Galactose/análogos & derivados; Conformação Proteica; Homologia de Sequência; Especificidade por Substrato; beta-Manosidase/química; beta-Manosidase/genética

Palavras-chave

GFPdomain fusion; affinity gel electrophoresis; enzyme kinetics; galactomannan; glucomannan; mannan; multimodular enzyme truncation; proteincarbohydrate interaction; pull-down assays; substrate specificity

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Celulose / Gammaproteobacteria / Beta-Manosidase / Mananas Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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