Inhibitors of heat shock protein 70 (Hsp70) with enhanced metabolic stability reduce tau levels.
Bioorg Med Chem Lett
; 41: 128025, 2021 06 01.
Article
em En
| MEDLINE
| ID: mdl-33839251
ABSTRACT
The molecular chaperone, Heat Shock Protein 70 (Hsp70), is an emerging drug target for neurodegenerative diseases, because of its ability to promote degradation of microtubule-associated protein tau (MAPT/tau). Recently, we reported YM-08 as a brain penetrant, allosteric Hsp70 inhibitor, which reduces tau levels. However, the benzothiazole moiety of YM-08 is vulnerable to metabolism by CYP3A4, limiting its further application as a chemical probe. In this manuscript, we designed and synthesized seventeen YM-08 derivatives by systematically introducing halogen atoms to the benzothiazole ring and shifting the position of the heteroatom in a distal pyridine. In microsome assays, we found that compound JG-23 has 12-fold better metabolic stability and it retained the ability to reduce tau levels in two cell-based models. These chemical probes of Hsp70 are expected to be useful tools for studying tau homeostasis.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas tau
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Proteínas de Choque Térmico HSP70
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Benzotiazóis
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Tiazolidinas
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article