Anti-C1-Inhibitor Autoantibody Detection by ELISA.

Suffritti, Chiara; Caccia, Sonia; Berra, Silvia; Parolin, Debora; Cicardi, Marco

Suffritti, Chiara; Caccia, Sonia; Berra, Silvia; Parolin, Debora; Cicardi, Marco.

Afiliação

Suffritti C; ASST-Fatebenefratelli-Sacco, Milan, Italy. chiarasuffritti@gmail.com.
Caccia S; Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Milan, Italy.
Berra S; Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Milan, Italy.
Parolin D; Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Milan, Italy.
Cicardi M; ASST-Fatebenefratelli-Sacco, Milan, Italy.

Methods Mol Biol ; 2227: 115-120, 2021.

Article em En | MEDLINE | ID: mdl-33847936

ABSTRACT

ABSTRACT

Enzyme-linked immunosorbent assay (ELISA) is a quantitative analytical method used to measure the concentration of molecules in biological fluids through antigen-antibody reactions. Here we describe the measurement of anti-C1-inhibitor autoantibodies by an indirect ELISA. In this method patients' sera are incubated in a microplate coated with plasma derived C1-inhibitor.

Assuntos

Autoanticorpos/análise; Proteínas Inativadoras do Complemento 1/imunologia; Angioedema/sangue; Angioedema/diagnóstico; Angioedema/imunologia; Angioedemas Hereditários/sangue; Angioedemas Hereditários/diagnóstico; Angioedemas Hereditários/imunologia; Animais; Autoanticorpos/sangue; Ensaio de Imunoadsorção Enzimática/métodos; Cabras; Humanos; Camundongos

Palavras-chave

Angioedema; Autoantibodies; C1-inhibitor; C1-inhibitor deficiency; ELISA

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Autoanticorpos / Proteínas Inativadoras do Complemento 1 Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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