Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes.
Methods Mol Biol
; 2281: 273-288, 2021.
Article
em En
| MEDLINE
| ID: mdl-33847965
ABSTRACT
Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall structural organization.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA de Cadeia Simples
/
Proteínas Mitocondriais
/
Proteínas de Ligação a DNA
/
Nucleoproteínas
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article