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Flos magnoliae constituent fargesin has an anti-allergic effect via ORAI1 channel inhibition.
Hong, Phan Thi Lam; Kim, Hyun Jong; Kim, Woo Kyung; Nam, Joo Hyun.
Afiliação
  • Hong PTL; Department of Physiology, Dongguk University College of Medicine, Gyeongju 38066, Korea.
  • Kim HJ; Channelopathy Research Center (CRC), Dongguk University College of Medicine, Goyang 10326, Korea.
  • Kim WK; Channelopathy Research Center (CRC), Dongguk University College of Medicine, Goyang 10326, Korea.
  • Nam JH; Channelopathy Research Center (CRC), Dongguk University College of Medicine, Goyang 10326, Korea.
Korean J Physiol Pharmacol ; 25(3): 251-258, 2021 May 01.
Article em En | MEDLINE | ID: mdl-33859065
Flos magnoliae (FM), the dry flower buds of Magnolia officinalis or its related species, is a traditional herbal medicine commonly used in Asia for symptomatic relief of and treating allergic rhinitis, headache, and sinusitis. Although several studies have reported the effects of FM on store-operated calcium entry (SOCE) via the ORAI1 channel, which is essential during intracellular calcium signaling cascade generation for T cell activation and mast cell degranulation, the effects of its isolated constituents on SOCE remain unidentified. Therefore, we investigated which of the five major constituents of 30% ethanoic FM (vanillic acid, tiliroside, eudesmin, magnolin, and fargesin) inhibit SOCE and their physiological effects on immune cells. The conventional whole-cell patch clamp results showed that fargesin, magnolin, and eudesmin significantly inhibited SOCE and thus human primary CD4+ T lymphocyte proliferation, as well as allergen-induced histamine release in mast cells. Among them, fargesin demonstrated the most potent inhibitory effects not only on ORAI1 (IC50 = 12.46 ± 1.300 µM) but also on T-cell proliferation (by 87.74% ± 1.835%) and mast cell degranulation (by 20.11% ± 5.366%) at 100 µM. Our findings suggest that fargesin can be a promising candidate for the development of therapeutic drugs to treat allergic diseases.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article