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Protein Delivery: If Your GFP (or Other Small Protein) Is in the Cytosol, It Will Also Be in the Nucleus.
Luther, David C; Jeon, Taewon; Goswami, Ritabrita; Nagaraj, Harini; Kim, Dongkap; Lee, Yi-Wei; Rotello, Vincent M.
Afiliação
  • Luther DC; Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
  • Jeon T; Molecular and Cellular Biology Graduate Program, University of Massachusetts, 230 Stockbridge Road, Amherst, Massachusetts 01003, United States.
  • Goswami R; Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
  • Nagaraj H; Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
  • Kim D; Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
  • Lee YW; Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea.
  • Rotello VM; Department of Chemistry, Hanyang University, Seoul 04763, Republic of Korea.
Bioconjug Chem ; 32(5): 891-896, 2021 05 19.
Article em En | MEDLINE | ID: mdl-33872490
Intracellular protein delivery is a transformative tool for biologics research and medicine. Delivery into the cytosol allows proteins to diffuse throughout the cell and access subcellular organelles. Inefficient delivery caused by endosomal entrapment is often misidentified as cytosolic delivery. This inaccuracy muddles what should be a key checkpoint in assessing delivery efficiency. Green fluorescent protein (GFP) is a robust cargo small enough to passively diffuse from the cytosol into the nucleus. Fluorescence of GFP in the nucleus is a direct readout for cytosolic access and effective delivery. Here, we highlight recent examples from the literature for the accurate assessment of cytosolic protein delivery using GFP fluorescence in the cytosol and nucleus.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Núcleo Celular / Citosol / Proteínas Luminescentes Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Núcleo Celular / Citosol / Proteínas Luminescentes Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article