Direct Transverse Relaxation Time Biosensing Strategy for Detecting Foodborne Pathogens through Enzyme-Mediated Sol-Gel Transition of Hydrogels.
Anal Chem
; 93(17): 6613-6619, 2021 05 04.
Article
em En
| MEDLINE
| ID: mdl-33886309
ABSTRACT
In this work, we develop a direct transverse relaxation time (T2) biosensing strategy and employ it for assaying foodborne pathogens relying on the alkaline phosphatase (ALP)-mediated sol-gel transition of hydrogels. ALP can catalyze the reaction to generate an acidic environment to transform the sol-state alginate solution to hydrogel, and this hydrogelation process can directly regulate the diffusion rate of water protons that results in a T2 change of water molecules. By means of enzyme-modulated sol-gel transition and antigen-antibody interactions, this T2 biosensor displays high sensitivity for detecting 50 CFU/mL S. enteritidis within 2 h. This biosensing strategy directly modulates the water molecules rather than magnetic probes in traditional methods, offering a straightforward, novel, and sensitive platform for pathogen detection.
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1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Hidrogéis
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article