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The regulatory role of miR-20a in bovine cumulus cells and its contribution to oocyte maturation.
Andreas, Eryk; Pandey, Hari Om; Hoelker, Michael; Salilew-Wondim, Dessie; Gebremedhn, Samuel; Schellander, Karl; Tesfaye, Dawit.
Afiliação
  • Andreas E; Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany.
  • Pandey HO; Robinson Research Institute, The University of Adelaide, 5000Adelaide, SA, Australia.
  • Hoelker M; Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany.
  • Salilew-Wondim D; Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany.
  • Gebremedhn S; Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany.
  • Schellander K; Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany.
  • Tesfaye D; Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, Colorado State University, 3105 Rampart Rd, Fort Collins, CO80521, USA.
Zygote ; 29(6): 435-444, 2021 Dec.
Article em En | MEDLINE | ID: mdl-33890561
ABSTRACT
Dynamic changes in microRNAs in oocyte and cumulus cells before and after maturation may explain the spatiotemporal post-transcriptional gene regulation within bovine follicular cells during the oocyte maturation process. miR-20a has been previously shown to regulate proliferation and differentiation as well as progesterone levels in cultured bovine granulosa cells. In the present study, we aimed to demonstrate the function of miR-20a during the bovine oocyte maturation process. Maturation of cumulus-oocyte complexes (COCs) was performed at 39°C in an humidified atmosphere with 5% CO2 in air. The expression of miR-20a was investigated in the cumulus cells and oocytes at 22 h post culture. The functional role of miR-20a was examined by modulating the expression of miR-20a in COCs during in vitro maturation (IVM). We found that the miR-20a expression was increased in cumulus cells but decreased in oocytes after IVM. Overexpression of miR-20a increased the oocyte maturation rate. Even though not statistically significant, miR-20a overexpression during IVM increased progesterone levels in the spent medium. This was further supported by the expression of STAR and CYP11A1 genes in cumulus cells. The phenotypes observed due to overexpression of miR-20a were validated by BMP15 supplementation during IVM and subsequent transfection of BMP15-treated COCs using miR-20a mimic or BMPR2 siRNA. We found that miR-20a mimic or BMPR2 siRNA transfection rescued BMP15-reduced oocyte maturation and progesterone levels. We concluded that miR-20a regulates oocyte maturation by increasing cumulus cell progesterone synthesis by simultaneous suppression of BMPR2 expression.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: MicroRNAs / Células do Cúmulo / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: MicroRNAs / Células do Cúmulo / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article