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Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli.
Madru, Clément; Tekpinar, Ayten Dizkirici; Rosario, Sandrine; Czernecki, Dariusz; Brûlé, Sébastien; Sauguet, Ludovic; Delarue, Marc.
Afiliação
  • Madru C; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
  • Tekpinar AD; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
  • Rosario S; Department of Molecular Biology and Genetics, Faculty of Science, Van Yüzüncü Yil University, Van, Turkey.
  • Czernecki D; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
  • Brûlé S; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
  • Sauguet L; École Doctorale Complexité du Vivant, Sorbonne Université, Paris, France.
  • Delarue M; Molecular Biophysics Platform, C2RT, Institut Pasteur, CNRS UMR, Paris, France.
PLoS One ; 16(4): e0250610, 2021.
Article em En | MEDLINE | ID: mdl-33914787
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clonagem Molecular / Proteínas não Estruturais Virais / Escherichia coli / RNA-Polimerase RNA-Dependente de Coronavírus / SARS-CoV-2 Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clonagem Molecular / Proteínas não Estruturais Virais / Escherichia coli / RNA-Polimerase RNA-Dependente de Coronavírus / SARS-CoV-2 Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article