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Gene Expression of Prox-1 and Hif-1a in Primo Vessels Inside Lymph Vessels of the Rabbit.
Shin, Jun-Young; Choi, Jong-Gu; Shin, Sungchul; Yeo, Sujung; Lee, Sang-Suk.
Afiliação
  • Shin JY; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Choi JG; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Shin S; Department of Animal Biotechnology, College of Biology Sciences, Sangji University, Wonju, Republic of Korea.
  • Yeo S; Department of Meridian and Acupuncture, College of Korean Medicine, Sangji University, Wonju, Republic of Korea.
  • Lee SS; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea. sslee@sangji.ac.kr.
Adv Exp Med Biol ; 1269: 387-391, 2021.
Article em En | MEDLINE | ID: mdl-33966247
The gene expression of Prox-1 and Hif-1a for the isolated primo vessels (PVs) and composite lymphatic vessels (LVs) containing PVs (LVs + PVs) was investigated by RNA-sequencing (Seq) and quantitative polymerase chain reaction (qRT-PCR) analysis. RNA-Seq on the passed 10 samples on RNA-QC for two experimental groups with PVs and PVs + LVs proceeded to the library construction stage automatically and analyzed differentially expressed genes (DEGs). From the real-time qRT-PCR analysis data, we found the marker genes of Prox-1 and Hif-1a were enriched and decreased in an isolated PVs compared to LVs, respectively. Based on mRNA transcriptional data, Prox-1 and Hif-1a were increased and decreased in PVs compared to LVs + PVs under lipopolysaccharide (LPS) treatment and relieved by acupuncture electric stimulation (AES), respectively. This finding indicates that high and low levels of Prox-1 and Hif-1a may be involved in the function of PVs and that pathophysiological and physiological condition could progress into inflamed lymphatic endothelial cells expanding the PV within the LV.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Endoteliais / Vasos Linfáticos Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Endoteliais / Vasos Linfáticos Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article