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JRAB/MICAL-L2 undergoes liquid-liquid phase separation to form tubular recycling endosomes.
Sakane, Ayuko; Yano, Taka-Aki; Uchihashi, Takayuki; Horikawa, Kazuki; Hara, Yusuke; Imoto, Issei; Kurisu, Shusaku; Yamada, Hiroshi; Takei, Kohji; Sasaki, Takuya.
Afiliação
  • Sakane A; Department of Biochemistry, Tokushima University Graduate School of Medical Sciences, Tokushima, Japan. sakane@tokushima-u.ac.jp.
  • Yano TA; Department of Interdisciplinary Researches for Medicine and Photonics, Institute of Post-LED Photonics, Tokushima, Japan. sakane@tokushima-u.ac.jp.
  • Uchihashi T; Department of Post-LED Photonics Research, Institute of Post-LED Photonics, Tokushima, Japan.
  • Horikawa K; Department of Physics, Nagoya University, Nagoya, Japan.
  • Hara Y; Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi, Japan.
  • Imoto I; Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, Tokushima, Japan.
  • Kurisu S; Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, Tokushima, Japan.
  • Yamada H; Division of Molecular Genetics, Aichi Cancer Center Research Institute, Nagoya, Japan.
  • Takei K; Department of Cancer Genetics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
  • Sasaki T; Department of Cell Biology, Tokushima University Graduate School of Medical Sciences, Tokushima, Japan.
Commun Biol ; 4(1): 551, 2021 05 11.
Article em En | MEDLINE | ID: mdl-33976349
ABSTRACT
Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated in tubulation of recycling endosomes, but the mechanism of endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces endosomal tubulation via activated Rab8A. In association with Rab8A, JRAB/MICAL-L2 adopts its closed form, which functions in the tubulation of recycling endosomes. Moreover, JRAB/MICAL-L2 induces liquid-liquid phase separation, initiating the formation of tubular recycling endosomes upon overexpression. Between its N-terminal and C-terminal globular domains, JRAB/MICAL-L2 contains an intrinsically disordered region, which contributes to the formation of JRAB/MICAL-L2 condensates. Based on our findings, we propose that JRAB/MICAL-L2 plays two sequential roles in the biogenesis of tubular recycling endosomes first, JRAB/MICAL-L2 organizes phase separation, and then the closed form of JRAB/MICAL-L2 formed by interaction with Rab8A promotes endosomal tubulation.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endossomos / Proteínas dos Microfilamentos Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Endossomos / Proteínas dos Microfilamentos Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article