Cyanine-Dyad Molecular Probe for the Simultaneous Profiling of the Evolution of Multiple Radical Species During Bacterial Infections.
Angew Chem Int Ed Engl
; 60(31): 16900-16905, 2021 07 26.
Article
em En
| MEDLINE
| ID: mdl-34018295
ABSTRACT
Real-time monitoring of the evolution of bacterial infection-associated multiple radical species is critical to accurately profile the pathogenesis and host-defense mechanisms. Here, we present a unique dual wavelength near-infrared (NIR) cyanine-dyad molecular probe (HCy5-Cy7) for simultaneous monitoring of reactive oxygen and nitrogen species (RONS) variations both inâ
vitro and inâ
vivo. HCy5-Cy7 specifically turns on its fluorescence at 660â
nm via superoxide or hydroxyl radical (O2.- , . OH)-mediated oxidation of reduced HCy5 moiety to Cy5, while peroxynitrite or hypochlorous species (ONOO- , ClO- )-induced Cy7 structural degradation causes the emission turn-off at 800â
nm. Such multispectral but reverse signal responses allow multiplex manifestation of inâ
situ oxidative and nitrosative stress events during the pathogenic and defensive processes in both bacteria-infected macrophage cells and living mice. Most importantly, this study may also provide new perspectives for understanding the bacterial pathogenesis and advancing the precision medicine against infectious diseases.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Infecções Bacterianas
/
Carbocianinas
/
Corantes
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article