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Label-free aptasensor for p24-HIV protein detection based on graphene quantum dots as an electrochemical signal amplifier.
Gogola, Jeferson L; Martins, Gustavo; Gevaerd, Ava; Blanes, Lucas; Cardoso, Josiane; Marchini, Fabricio Klerynton; Banks, Craig E; Bergamini, Márcio F; Marcolino-Junior, Luiz H.
Afiliação
  • Gogola JL; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal Do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Martins G; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal Do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Gevaerd A; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal Do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Blanes L; Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba, Brazil.
  • Cardoso J; Paraná Institute of Molecular Biology, Curitiba, Brazil.
  • Marchini FK; Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba, Brazil.
  • Banks CE; Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street Manchester M1 5GD, UK.
  • Bergamini MF; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal Do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil.
  • Marcolino-Junior LH; Laboratório de Sensores Eletroquímicos (LabSensE), Departamento de Química, Universidade Federal Do Paraná (UFPR), CP 19032, CEP, 81531-990 Curitiba, PR, Brazil. Electronic address: luiz1berto@ufpr.br.
Anal Chim Acta ; 1166: 338548, 2021 Jun 29.
Article em En | MEDLINE | ID: mdl-34022998
Human immunodeficiency virus (HIV) is still considered a pandemic, and the detection of p24-HIV protein has an important role in the early diagnosis of HIV in adults and newborns. The accessibility of these trials depends on the price and execution difficulty of the method, which can be reduced using electrochemical methods by using enzymeless approaches, disposable and accurate devices. In this work, graphene quantum dots were acquired by a simple synthesis and employed as an electrochemical signal amplifier and support for the aptamer immobilization through a feasible and stable modification of disposable screen-printed electrodes. The device has been easily assembled and used to detect p24-HIV protein without the interference of similar proteins or sample matrix. Using the best set of experimental conditions, a linear correlation between analytical signal and log of p24-HIV concentration from 0.93 ng mL-1 to 93 µg mL-1 and a limit of detection of 51.7 pg mL-1 were observed. The developed device was applied to p24 determination in spiked human serum and provided distinct levels of signal for positive and negative samples, successfully identifying real samples with the target protein. This sensor is a step towards the development of point-of-care devices and the popularization of electrochemical methods for trials and diagnostics of relevant diseases.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Infecções por HIV / Pontos Quânticos / Aptâmeros de Nucleotídeos / Grafite Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adult / Humans / Newborn Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Infecções por HIV / Pontos Quânticos / Aptâmeros de Nucleotídeos / Grafite Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adult / Humans / Newborn Idioma: En Ano de publicação: 2021 Tipo de documento: Article