Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for <i>in vivo</i> wide-field imaging of the mouse brain.

Takahashi, Taiga; Zhang, Hong; Otomo, Kohei; Okamura, Yosuke; Nemoto, Tomomi

Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain.

Takahashi, Taiga; Zhang, Hong; Otomo, Kohei; Okamura, Yosuke; Nemoto, Tomomi.

Afiliação

Takahashi T; Biophotonics Research Group, Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Zhang H; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Otomo K; School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Higashiyama 5-1, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Okamura Y; Department of Applied Chemistry, School of Engineering, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan.
Nemoto T; Micro/Nano Technology Center, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan.

STAR Protoc ; 2(2): 100542, 2021 06 18.

Article em En | MEDLINE | ID: mdl-34027495

Assuntos

Encéfalo/diagnóstico por imagem; Nanoestruturas/química; Imagem Óptica/métodos; Crânio/cirurgia; Animais; Camundongos; Microscopia de Fluorescência por Excitação Multifotônica; Polietilenoglicóis/química

Palavras-chave

Microscopy; Neuroscience

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Crânio / Encéfalo / Nanoestruturas / Imagem Óptica Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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