Comparison of the frequency of periodontal pathogenic species of diabetics and non-diabetics and its relation to periodontitis severity, glycemic control and body mass index.

ABSTRACT

OBJECTIVES: To investigate common pathogenic bacteria of periodontal diseases (PD) in patients with type 2 diabetes mellitus (T2DM) and its relationship with PD severity, glycemic control and body mass index (BMI). MATERIAL AND METHODS: This case-control study consisted of 55 patients with T2DM and 55 individuals as control. Samples were collected from periodontal pockets. After DNA extraction, using 16srRNA-specific primers, the presence of Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Prevotella intermedia (Pi), and Fusobacterium nucleatum (Fn) were examined based on polymerase chain reaction method. RESULTS: Aa frequency was significantly higher in in T2DM group than control. There were no significant differences in the frequencies of Pg, Tf, Pi, and Fn between studied groups. There were no significant differences between frequencies of studied bacteria in different severities of periodontitis in T2DM group. Prevalence of Tf in T2DM patients with moderate periodontitis was significantly higher than non-diabetics with moderate periodontitis. There was no significant difference between the frequency of bacteria in diabetics with good and poor glycemic control. There was a significant difference between the frequencies of Pg in T2DM individuals with different BMI levels. CONCLUSIONS: A higher frequency of detection of Aa was found in diabetic when compared to non-diabetics. Glycemic control did not affect the frequency distribution of studied bacteria in T2DM. Pg was identified in higher frequency in overweight T2DM patients.