Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cells.
Methods Mol Biol
; 2308: 3-20, 2021.
Article
em En
| MEDLINE
| ID: mdl-34057710
ABSTRACT
Mesenchymal stromal cells (MSC) are a rare, heterogeneous and multipotent population that can be isolated from several tissues. MSC were originally discovered in the bone marrow and studied for their capacity to maintain hematopoietic cells. We will describe here methods to isolate, culture, and bank MSC from human bone marrow. Then, characterization protocols by flow cytometry, clonogenic assays and doubling time evaluation will be developed. Finally, in vitro MSC culture and differentiation into osteoblasts, adipocytes, and chondrocytes will be explained. Thus, this chapter will detail all bases to work on MSC with consensus and clear methods and protocols.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Proliferação de Células
/
Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article