Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition.
Int J Mol Sci
; 22(11)2021 May 24.
Article
em En
| MEDLINE
| ID: mdl-34074049
ABSTRACT
The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in Arabidopsis thaliana. The over-expression of miR156 in transgenic Populus × canadensis dramatically prolongs the juvenile phase. Here, we find that the expression of mno-miR156 decreases with age in all tissues in mulberry, which led us to study the hierarchical action of miR156 in mulberry. Utilizing degradome sequencing and dual-luciferase reporter assays, nine MnSPLs were shown to be directly regulated by miR156. The results of yeast one-hybrid and dual-luciferase reporter assays also revealed that six MnSPLs could recognize the promoter sequences of mno-miR172 and activate its expression. Our results demonstrate that mno-miR156 performs its role by repressing MnSPL/mno-miR172 pathway expression in mulberry. This work uncovered a miR156/SPLs/miR172 regulation pathway in the development of mulberry and fills a gap in our knowledge about the molecular mechanism of vegetative phase transition in perennial woody plants.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Envelhecimento
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Arabidopsis
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Morus
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MicroRNAs
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article