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Screening of Biological Target Molecules Related to Glucocorticoid-Induced Cataract (GIC) on the Basis of Constructing ceRNA Network.
Shi, Zhan; Zhao, Xiumei; Su, Ying; Wang, Chao; Liu, Ping; Ge, Hongyan.
Afiliação
  • Shi Z; Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, No. 23 Post Street, Nangang District, Harbin City, Heilongjiang Province, China.
  • Zhao X; Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, No. 23 Post Street, Nangang District, Harbin City, Heilongjiang Province, China.
  • Su Y; Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, No. 23 Post Street, Nangang District, Harbin City, Heilongjiang Province, China.
  • Wang C; Department of Anesthesiolgy, The First Affiliated Hospital, Harbin Medical University, No. 23 Post Street, Nangang District, Harbin City, Heilongjiang Province, China.
  • Liu P; Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, No. 23 Post Street, Nangang District, Harbin City, Heilongjiang Province, China. Ping_liu1954@163.com.
  • Ge H; Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, No. 23 Post Street, Nangang District, Harbin City, Heilongjiang Province, China. gehongyan@hrbmu.edu.cn.
Biochem Genet ; 60(1): 24-38, 2022 Feb.
Article em En | MEDLINE | ID: mdl-34076796
Glucocorticoid-induced cataract (GIC)-associated biomarkers were screened by ceRNA network construction. The GIC samples' GSE3040 were obtained from the NCBI-GEO database. R's Limma package was used to identify differentially expressed RNAs (DERs) between the normal and GIC samples group (4- and 16-h). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis for the mRNAs in the constructed GIC lncRNA-miRNA-mRNA ceRNA regulation network was implemented. A total of 1665 and 1443 DERs were obtained in the 4- and 16-h group, respectively. At two time points, 256 overlapping DERs were identified, of which 210 (17 lncRNAs and 203 mRNAs) had significant differential expression (4 down- and 206 up-regulated). A total of 534 co-expressed ligation pairs (all up-regulated) were obtained. A ceRNA regulation network was constructed. RPS6KA5, GAB1, CCR7, CCL2, COL4A4, and PPARG were obtained and significantly enriched in the 4 KEGG signaling pathways and were featured as GIC target molecules.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Catarata / MicroRNAs / RNA Longo não Codificante Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Catarata / MicroRNAs / RNA Longo não Codificante Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article