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Linc-KIAA1737-2 promoted LPS-induced HK-2 cell apoptosis by regulating miR-27a-3p/TLR4/NF-κB axis.
Hu, Ming; Wei, Jing; Yang, Liu; Xu, Jianhua; He, Zhaofeng; Li, Haiyuan; Ning, Chao; Lu, Shijun.
Afiliação
  • Hu M; Department of Anesthesiology, Sir Run Run Shaw Hospital, Zhejiang University, School of Medicine, Hangzhou, China.
  • Wei J; Department of General practice, Qingdao Ninth People's Hospital, 2th Chaocheng Road, Qingdao, Shandong, People's Republic of China.
  • Yang L; Department of Neurology, Chongqing Emergency Medical Center, The Affiliated Central Hospital to Chongqing University, Chongqing, China.
  • Xu J; Department of Critical Care Medicine, Shandong Province Linyi Central Hospital, Linyi, Shandong, China.
  • He Z; Department of Critical Care Medicine, Shandong Province Juxian People's Hospital, Juxian, Shandong, China.
  • Li H; Department of Critical Care Medicine, Shandong Province Linyi Central Hospital, Linyi, Shandong, China.
  • Ning C; Department of Critical Care Medicine, Shandong Province Linyi Central Hospital, Linyi, Shandong, China.
  • Lu S; Department of Critical Care Medicine, Shandong Province Linyi Central Hospital, Linyi, Shandong, China. lushijun2020@126.com.
J Bioenerg Biomembr ; 53(4): 393-403, 2021 08.
Article em En | MEDLINE | ID: mdl-34076840
Inflammation and renal cell apoptosis participate in sepsis-induced acute kidney injury. Previous research found the upregulation of long non-coding RNA Linc-KIAA1737-2 in hypoxia- or inflammation-challenged human proximal tubular epithelial cells, but its role in sepsis-induced acute kidney injury is underexplored. In this research, we found that Linc-KIAA1737-2 could be upregulated in HK-2 human proximal tubular epithelial cells by LPS treatment, and knock-down of this lncRNA significantly attenuated LPS-induced apoptosis in HK-2 cells, while its overexpression showed opposite effect. MiR-27a-3p was confirmed to interact with Linc-KIAA1737-2 in HK-2 cells by RNA pull-down and dual-luciferase assay. MiR-27a-3p mimic transfection significantly attenuated LPS-induced HK-2 cell apoptosis by downregulating the protein levels of TLR4 and NF-κB, which was overturned by overexpression of Linc-KIAA1737-2. Our results suggested that Linc-KIAA1737-2 could promote LPS-induced apoptosis in HK-2 cells, and presumably sepsis-induced acute kidney injury, by regulating the miR-27a-3p/TLR4/NF-κB axis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Lipopolissacarídeos / NF-kappa B / MicroRNAs / RNA Longo não Codificante Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Lipopolissacarídeos / NF-kappa B / MicroRNAs / RNA Longo não Codificante Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article