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Individual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer.
Glatthorn, Haley N; Hanson, Brent M; Kim, Julia G; Herlihy, Nola S; Klimczak, Amber M; Hong, Kathleen H; Seli, Emre; Scott, Richard T.
Afiliação
  • Glatthorn HN; Department of Obstetrics, Gynecology, and Reproductive Sciences, Rutgers Robert Wood Johnson Medical School, 125 Paterson Street, Suite 2100, New Brunswick, NJ, 08901, USA. hg299@rwjms.rutgers.edu.
  • Hanson BM; IVI-Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ, USA.
  • Kim JG; Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
  • Herlihy NS; IVI-Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ, USA.
  • Klimczak AM; Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
  • Hong KH; IVI-Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ, USA.
  • Seli E; Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
  • Scott RT; IVI-Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ, USA.
J Assist Reprod Genet ; 38(8): 2157-2164, 2021 Aug.
Article em En | MEDLINE | ID: mdl-34086147
PURPOSE: To evaluate embryology and pregnancy outcomes following individual and group embryo culture in the setting of contemporary laboratory practices and freeze-all cycles. METHODS: Patients underwent ovarian stimulation followed by intracytoplasmic sperm injection (ICSI). Embryos proceeded through individual culture and then underwent preimplantation genetic testing for aneuploidy (PGT-A) via trophectoderm biopsy. In a subsequent cycle, participants underwent single embryo transfer of a vitrified-warmed, euploid embryo. Outcomes were compared to controls undergoing group culture during the same time frame. The Mann-Whitney U test and logistic regression models were utilized. RESULTS: Outcomes were assessed for 144 patients whose embryos underwent individual culture and 449 controls whose embryos underwent group culture. There were no significant differences in fertilization rates between groups (81.7% for individual culture vs. 84.1% for group culture, p = 0.22). However, individual culture was associated with a decreased rate of blastocyst formation compared to group culture (43.5% vs. 48.5%, p < 0.01). Following single, vitrified-warmed euploid blastocyst transfer, there were no significant differences between individual culture and group culture, respectively, in rates of positive ßhCG (81.9% vs. 81.5%, p = 0.91), sustained implantation (63.9% vs. 65.0%, p = 0.80), biochemical miscarriage (16.7% vs. 12.3%, p = 0.18), or clinical miscarriage (1.4% vs. 4.2%, p = 0.13). CONCLUSION: While individual culture appears to negatively impact the rate of usable blastocyst formation compared to group culture, there were no significant differences in pregnancy outcomes following transfer of a single, vitrified-warmed euploid blastocyst.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Blastocisto / Fertilização in vitro / Injeções de Esperma Intracitoplásmicas / Técnicas de Cultura Embrionária / Transferência Embrionária / Vitrificação Tipo de estudo: Diagnostic_studies / Observational_studies / Risk_factors_studies Limite: Adolescent / Adult / Female / Humans / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Blastocisto / Fertilização in vitro / Injeções de Esperma Intracitoplásmicas / Técnicas de Cultura Embrionária / Transferência Embrionária / Vitrificação Tipo de estudo: Diagnostic_studies / Observational_studies / Risk_factors_studies Limite: Adolescent / Adult / Female / Humans / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article