Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach.
Stem Cell Res
; 53: 102380, 2021 05.
Article
em En
| MEDLINE
| ID: mdl-34088009
ABSTRACT
Epstein-Barr virus (EBV)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (iPSCs) without viral transduction. Here, we report establishment of an iPSC line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; Callithrix jacchus) using an all-in-one episomal vector that we newly developed. The established iPSC line, named NM-iPS, showed standard characteristics of pluripotency such as pluripotency-related marker expression, three germ layer differentiation, and normal karyotype (2n = 46). The novel iPSC line would be a useful resource for stem cell research using non-human primates.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Infecções por Vírus Epstein-Barr
/
Células-Tronco Pluripotentes Induzidas
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article