Characterisation of microglia isolated from adult human and rat brain.
J Neuroimmunol
; 19(3): 177-89, 1988 Sep.
Article
em En
| MEDLINE
| ID: mdl-3410964
ABSTRACT
A method has been developed to isolate microglia from adult human and rat brain cell suspensions by rosette formation via Fc receptors. Immunocytochemical characterisation of the cells immediately following isolation and after 7-10 days in vitro with a panel of monoclonal antibodies has demonstrated that microglia from adult brain have the phenotypic characteristics and phagocytic capacity of mononuclear phagocytes, but lack the hydrolytic enzyme, non-specific esterase. The ability to isolate rapidly a purified population of microglia from adult brain provides a means for investigating mechanisms of activation and differentiation of tissue macrophages, which could elucidate their role in inflammation of the central nervous system.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Neuroglia
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article