H3.3 kinetics predicts chromatin compaction status of parental genomes in early embryos.
Reprod Biol Endocrinol
; 19(1): 87, 2021 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-34116678
ABSTRACT
BACKGROUND:
After fertilization, the fusion of gametes results in the formation of totipotent zygote. During sperm-egg fusion, maternal factors participate in parental chromatin remodeling. H3.3 is a histone H3 variant that plays essential roles in mouse embryogenesis.METHODS:
Here, we used transgenic early embryos expressing H3.3-eGFP or H2B-mCherry to elucidate changes of histone mobility.RESULTS:
We used FRAP analysis to identify that maternally stored H3.3 has a more significant change than H2B during maternal-to-embryonic transition. We also found that H3.3 mobile fraction, which may be regulated by de novo H3.3 incorporation, reflects chromatin compaction of parental genomes in GV oocytes and early embryos.CONCLUSIONS:
Our results show that H3.3 kinetics in GV oocytes and early embryos is highly correlated with chromatin compaction status of parental genomes, indicating critical roles of H3.3 in higher-order chromatin organization.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Histonas
/
Genoma
/
Desenvolvimento Embrionário
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article