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GLUT5 is a determinant of dietary fructose-mediated exacerbation of experimental colitis.
Basu, Srijani; Liu, Catherine; Zhou, Xi Kathy; Nishiguchi, Ryohei; Ha, Taehoon; Chen, Justin; Johncilla, Melanie; Yantiss, Rhonda K; Montrose, David C; Dannenberg, Andrew J.
Afiliação
  • Basu S; Department of Medicine, Weill Cornell Medicine, New York, New York.
  • Liu C; Department of Medicine, Weill Cornell Medicine, New York, New York.
  • Zhou XK; Department of Population Health Sciences, Weill Cornell Medicine, New York, New York.
  • Nishiguchi R; Department of Medicine, Weill Cornell Medicine, New York, New York.
  • Ha T; Department of Population Health Sciences, Weill Cornell Medicine, New York, New York.
  • Chen J; Department of Medicine, Weill Cornell Medicine, New York, New York.
  • Johncilla M; Department of Pathology & Laboratory Medicine, Weill Cornell Medicine, New York, New York.
  • Yantiss RK; Department of Pathology & Laboratory Medicine, Weill Cornell Medicine, New York, New York.
  • Montrose DC; Department of Pathology, Renaissance School of Medicine, Stony Brook University, Stony Brook, New York.
  • Dannenberg AJ; Stony Brook Cancer Center, Stony Brook, New York.
Am J Physiol Gastrointest Liver Physiol ; 321(2): G232-G242, 2021 08 01.
Article em En | MEDLINE | ID: mdl-34133236
The Western diet has been suggested to contribute to the rising incidence of inflammatory bowel diseases. This has led to the hypothesis that fructose, a component of the Western diet, could play a role in the pathogenesis of inflammatory bowel diseases. A high-fructose diet is known to exacerbate experimental colitis. This study tested whether the expression of GLUT5, the fructose transporter, is a determinant of the severity of experimental colitis during elevated fructose consumption and whether ileal inflammation is associated with altered GLUT5 expression in Crohn's disease. Studies in genetically engineered mice showed that in comparison to Glut5+/+ mice, feeding a 15 kcal% fructose diet to Glut5-/- mice led to worse dextran sodium sulfate (DSS)-induced colitis. This effect was associated with elevated levels of colonic fructose and a shift in the fecal microbiota in Glut5-/- mice. Importantly, treatment with broad-spectrum antibiotics protected against the worsening of colitis mediated by dietary fructose in Glut5-/- mice. Gene expression analysis revealed that GLUT5 levels are reduced in the intestines of patients with ileal Crohn's disease. Moreover, levels of GLUT5 negatively correlated with expression of proinflammatory mediators in these samples. Collectively, these results demonstrate that dietary constituent (fructose)-host gene (GLUT5) interactions can shape the colonic microbiota, thereby impacting the severity of colitis.NEW & NOTEWORTHY This study provides the first evidence that reduced levels of GLUT5, the fructose transporter, worsen experimental colitis upon fructose feeding, an effect mediated by changes in the gut microbiota. Moreover, GLUT5 expression is reduced in Crohn's ileitis. Overall, these findings demonstrate the importance of interactions between dietary fructose and host GLUT5 as determinants of both the composition of colonic microbiota and severity of experimental colitis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Doença de Crohn / Transportador de Glucose Tipo 5 / Frutose Tipo de estudo: Etiology_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Doença de Crohn / Transportador de Glucose Tipo 5 / Frutose Tipo de estudo: Etiology_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article