Determination of protein concentration in downstream biomanufacturing processes by in-line index of refraction.
Biotechnol Prog
; 37(5): e3187, 2021 09.
Article
em En
| MEDLINE
| ID: mdl-34164947
ABSTRACT
Protein concentration determination is a necessary in-process control for the downstream operations within biomanufacturing. As production transitions from batch mode to an integrated continuous bioprocess paradigm, there is a growing need to move protein concentration quantitation from off-line to in-line analysis. One solution to fulfill this process analytical technology need is an in-line index of refraction (IoR) sensor to measure protein concentration in real time. Here the performance of an IoR sensor is evaluated through a series of experiments to assess linear response, buffer matrix effects, dynamic range, sensor-to-sensor variability, and the limits of detection and quantitation. The performance of the sensor was also tested in two bioprocessing scenarios, ultrafiltration and capture chromatography. The implementation of this in-line IoR sensor for real-time protein concentration analysis and monitoring has the potential to improve continuous bioprocess manufacturing.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Refratometria
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Proteínas Recombinantes
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Reatores Biológicos
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Anticorpos Monoclonais
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article