Development of a Highly Sensitive ß-Glucan Detection System Using Scanning Single-Molecule Counting Method.
Int J Mol Sci
; 22(11)2021 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-34205910
ABSTRACT
To overcome the limitations of the Limulus amebocyte lysate (LAL) assay method for the diagnosis of invasive fungal infection, we applied a reaction system combining recombinant ß-glucan binding proteins and a scanning single-molecule counting (SSMC) method. A novel (1â3)-ß-D-glucan recognition protein (S-BGRP) and a (1â6)-ß-glucanase mutant protein were prepared and tested for the binding of (1â6)-branched (1â3)-ß-D-glucan from fungi. S-BGRP and (1â6)-ß-glucanase mutant proteins reacted with ß-glucan from Candida and Aspergillus spp. Although LAL cross-reacted with plant-derived ß-glucans, the new detection system using the SSMC method showed low sensitivity to plant (1â3)-ß-D-glucan, which significantly improved the appearance of false positives, a recognized problem with the LAL method. Measurement of ß-glucan levels by the SSMC method using recombinant ß-glucan-binding proteins may be useful for the diagnosis of fungal infections. This study shows that this detection system could be a new alternative diagnostic method to the LAL method.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Beta-Glucanas
/
Endotoxinas
/
Micoses
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article