Microtubule inhibitors identified through nonbiased screening enhance DNA transfection efficiency by delaying p62-dependent ubiquitin recruitment.

Tsuchiya, Megumi; Ogawa, Hidesato; Watanabe, Kento; Koujin, Takako; Mori, Chie; Nunomura, Kazuto; Lin, Bangzhong; Tani, Akiyoshi; Hiraoka, Yasushi; Haraguchi, Tokuko

Tsuchiya, Megumi; Ogawa, Hidesato; Watanabe, Kento; Koujin, Takako; Mori, Chie; Nunomura, Kazuto; Lin, Bangzhong; Tani, Akiyoshi; Hiraoka, Yasushi; Haraguchi, Tokuko.

Afiliação

Tsuchiya M; Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Ogawa H; Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Watanabe K; Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Koujin T; Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Mori C; Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Nunomura K; Graduate School of Pharmaceutical Science, Osaka University, Suita, Japan.
Lin B; Graduate School of Pharmaceutical Science, Osaka University, Suita, Japan.
Tani A; Graduate School of Pharmaceutical Science, Osaka University, Suita, Japan.
Hiraoka Y; Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Haraguchi T; Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.

Genes Cells ; 26(9): 739-751, 2021 Sep.

Article em En | MEDLINE | ID: mdl-34212463

Assuntos

Microtúbulos/efeitos dos fármacos; Bibliotecas de Moléculas Pequenas/farmacologia; Transfecção/métodos; Moduladores de Tubulina/farmacologia; Ubiquitinação; Animais; Células Cultivadas; Colchicina/farmacologia; Endocitose; Fibroblastos/efeitos dos fármacos; Fibroblastos/metabolismo; Ensaios de Triagem em Larga Escala/métodos; Camundongos; Microtúbulos/metabolismo; Proteína Sequestossoma-1/metabolismo; Ubiquitina/metabolismo; Vimblastina/farmacologia

Palavras-chave

autophagy; gene delivery; high-throughput screening; p62; phosphorylation; transfection efficiency; ubiquitination

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transfecção / Moduladores de Tubulina / Bibliotecas de Moléculas Pequenas / Ubiquitinação / Microtúbulos Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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