X-ray Laue diffraction from crystals of xylose isomerase.
Proc Natl Acad Sci U S A
; 85(1): 112-5, 1988 Jan.
Article
em En
| MEDLINE
| ID: mdl-3422408
ABSTRACT
The Laue method (stationary crystal, polychromatic x-rays) was used to collect native and heavy-atom-derivative data on crystals of xylose isomerase (EC 5.3.1.5). These data were used to find the heavy-atom positions. The positions found by use of Laue data are the same as those found by use of monochromatic data collected on a diffractometer. These results confirm that Laue diffraction data sets, which can be obtained on a millisecond time scale, can be used to locate small molecules bound to protein active sites. The successful determination of heavy-atom positions also indicates that x-ray crystallographic data collected by the Laue method can be used to solve protein structures.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carboidratos Epimerases
/
Aldose-Cetose Isomerases
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article