Structure-selective differentiation of deletion mutations in circulating tumor DNA using dual probe-based isothermal amplification.
Chem Commun (Camb)
; 57(55): 6796-6799, 2021 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-34236364
ABSTRACT
A rapid dual probe-based fluorimetric assay was developed to detect deletion mutations in circulating tumor DNA using structure-selective isothermal amplification and pattern recognition. This method could detect both homozygous and heterozygous deletion configurations in a one-set experiment and achieved picomolar detection limits with high selectivity within 2 hours. It was promising for point-of-care cancer diagnosis in hospital settings.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Deleção de Sequência
/
Técnicas de Amplificação de Ácido Nucleico
/
DNA Tumoral Circulante
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article