Characterization of resistance mechanisms of Enterobacter cloacae Complex co-resistant to carbapenem and colistin.
BMC Microbiol
; 21(1): 208, 2021 07 08.
Article
em En
| MEDLINE
| ID: mdl-34238225
ABSTRACT
BACKGROUND:
The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in Enterobacter cloacae Complex (ECC) strains.RESULTS:
This study showed that the mechanisms of carbapenem resistance in this study are 1. Generating carbapenemase (7 of 19); 2. The production of AmpC or ESBLs combined with decreased expression of out membrane protein (12 of 19). hsp60 sequence analysis suggested 10 of 19 the strains belong to colistin hetero-resistant clusters and the mechanism of colistin resistance is increasing expression of acrA in the efflux pump AcrAB-TolC alone (18 of 19) or accompanied by a decrease of affinity between colistin and outer membrane caused by the modification of lipid A (14 of 19). Moreover, an ECC strain co-harboring plasmid-mediated mcr-4.3 and blaNDM-1 has been found.CONCLUSIONS:
This study suggested that there is no overlap between the resistance mechanism of co-resistant ECC strains to carbapenem and colistin. However, the emergence of strain co-harboring plasmid-mediated resistance genes indicated that ECC is a potential carrier for the horizontal spread of carbapenems and colistin resistance.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Carbapenêmicos
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Enterobacter cloacae
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Colistina
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Farmacorresistência Bacteriana
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Enterobacteriáceas Resistentes a Carbapenêmicos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article