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Evaluating the use of piezo manipulator, laser or their combination for blastocoel cavity puncture to improve cryopreservation outcomes of large equine embryos.
Rajabi-Toustani, Reza; Watanabe, Hiroyuki; Tsogtgerel, Munkhtuul; Gao, Yuanzhi; Canbo, Li; Haneda, Shingo; Cheong, Soon Hon; Nambo, Yasuo.
Afiliação
  • Rajabi-Toustani R; Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Watanabe H; Department of Life and Food Sciences, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Tsogtgerel M; Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Gao Y; United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
  • Canbo L; Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Haneda S; Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Cheong SH; Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
  • Nambo Y; Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
Reprod Domest Anim ; 56(10): 1358-1362, 2021 Oct.
Article em En | MEDLINE | ID: mdl-34254367
The main difficulty of large equine embryo cryopreservation is the replacement of blastocoel fluid with cryoprotectant solution. The objective of this study was to improve the cryopreservation of large equine embryos with PMAP and/or LAP. Embryos were collected via the non-surgical transcervical procedure and divided into three groups based on their size (A ≤ 300 µm, 300 µm300 µm). However, more research is required to find the best method for embryos ≥700 µm.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transferência Embrionária / Cavalos / Lasers / Micromanipulação Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transferência Embrionária / Cavalos / Lasers / Micromanipulação Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2021 Tipo de documento: Article