Nonspecific Synthesis in the Reactions of Isothermal Nucleic Acid Amplification.
Biochemistry (Mosc)
; 86(7): 887-897, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-34284713
ABSTRACT
The review focuses on the main factors involved in the formation of nonspecific products in isothermal nucleic acid amplification, such as mispriming, ab initio DNA synthesis, and additional activities of DNA polymerases, and discusses approaches to prevent formation of such nonspecific products in LAMP, RPA, NASBA, RCA, SDA, LSDA, NDA, and EXPAR.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
Técnicas de Amplificação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article