Generation of microRNA-378a-deficient hiPSC as a novel tool to study its role in human cardiomyocytes.

Martyniak, Alicja; Andrysiak, Kalina; Motais, Benjamin; Coste, Solène; Podkalicka, Paulina; Ferdek, Pawel; Stepniewski, Jacek; Dulak, Józef

Martyniak, Alicja; Andrysiak, Kalina; Motais, Benjamin; Coste, Solène; Podkalicka, Paulina; Ferdek, Pawel; Stepniewski, Jacek; Dulak, Józef.

Afiliação

Martyniak A; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Andrysiak K; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Motais B; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Coste S; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Podkalicka P; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Ferdek P; Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Stepniewski J; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland. Electronic address: jacek.stepniewski@uj.edu.pl.
Dulak J; Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland. Electronic address: jozef.dulak@uj.edu.pl.

J Mol Cell Cardiol ; 160: 128-141, 2021 11.

Article em En | MEDLINE | ID: mdl-34329686

Assuntos

Sinalização do Cálcio/genética; Células-Tronco Pluripotentes Induzidas/citologia; Células-Tronco Pluripotentes Induzidas/metabolismo; MicroRNAs/metabolismo; Miócitos Cardíacos/citologia; Miócitos Cardíacos/metabolismo; Antagomirs/genética; Cálcio/metabolismo; Cardiomiopatias/complicações; Cardiomiopatias/genética; Cardiomiopatias/metabolismo; Diferenciação Celular/genética; Crescimento Celular; Tamanho Celular; Distrofina/genética; Distrofina/metabolismo; Éxons; Deleção de Genes; Células HEK293; Humanos; MicroRNAs/genética; Distrofia Muscular de Duchenne/sangue; Distrofia Muscular de Duchenne/complicações; Distrofia Muscular de Duchenne/genética; Receptor IGF Tipo 1/metabolismo; Transfecção

Palavras-chave

CRISPR/Cas9; Cardiomyocytes; Duchenne muscular dystrophy; Human induced pluripotent stem cells; microRNA-378a

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sinalização do Cálcio / Miócitos Cardíacos / MicroRNAs / Células-Tronco Pluripotentes Induzidas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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