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The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival.
Steffen, Philipp A; Altmutter, Christina; Dworschak, Eva; Junttila, Sini; Gyenesei, Attila; Zhu, Xinzhou; Kockmann, Tobias; Ringrose, Leonie.
Afiliação
  • Steffen PA; Institute of Molecular Biotechnology (IMBA), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
  • Altmutter C; Institute of Molecular Biotechnology (IMBA), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
  • Dworschak E; Institute of Molecular Biotechnology (IMBA), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
  • Junttila S; Vienna Biocenter Core Facilities GmbH (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
  • Gyenesei A; Vienna Biocenter Core Facilities GmbH (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
  • Zhu X; Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058, Basel, Switzerland.
  • Kockmann T; Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058, Basel, Switzerland.
  • Ringrose L; Institute of Molecular Biotechnology (IMBA), Dr. Bohr-Gasse 3, 1030, Vienna, Austria. leonie.ringrose@hu-berlin.de.
Chromosoma ; 130(2-3): 215-234, 2021 09.
Article em En | MEDLINE | ID: mdl-34331109
ABSTRACT
The Drosophila Trithorax group (TrxG) protein ASH1 remains associated with mitotic chromatin through mechanisms that are poorly understood. ASH1 dimethylates histone H3 at lysine 36 via its SET domain. Here, we identify domains of the TrxG protein ASH1 that are required for mitotic chromatin attachment in living Drosophila. Quantitative live imaging demonstrates that ASH1 requires AT hooks and the BAH domain but not the SET domain for full chromatin binding in metaphase, and that none of these domains are essential for interphase binding. Genetic experiments show that disruptions of the AT hooks and the BAH domain together, but not deletion of the SET domain alone, are lethal. Transcriptional profiling demonstrates that intact ASH1 AT hooks and the BAH domain are required to maintain expression levels of a specific set of genes, including several involved in cell identity and survival. This study identifies in vivo roles for specific ASH1 domains in mitotic binding, gene regulation, and survival that are distinct from its functions as a histone methyltransferase.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Cromatina / Proteínas de Drosophila / Proteínas de Ligação a DNA / Drosophila Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Cromatina / Proteínas de Drosophila / Proteínas de Ligação a DNA / Drosophila Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article