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Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages.
Yang, Fan; Andersen, Daniel S; Trabue, Steven; Kent, Angela D; Pepple, Laura M; Gates, Richard S; Howe, Adina S.
Afiliação
  • Yang F; Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, United States of America.
  • Andersen DS; Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, United States of America.
  • Trabue S; USDA-Agricultural Research Service, National Laboratory for Agriculture and the Environment, Ames, Iowa, United States of America.
  • Kent AD; The Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
  • Pepple LM; The Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
  • Gates RS; Egg Industry Center, Iowa State University, Ames, Iowa, United States of America.
  • Howe AS; Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, United States of America.
PLoS One ; 16(8): e0254730, 2021.
Article em En | MEDLINE | ID: mdl-34343206
Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit's microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities' role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbiota / Esterco / Metano Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbiota / Esterco / Metano Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article