Protein interaction landscapes revealed by advanced in vivo cross-linking-mass spectrometry.

Wheat, Andrew; Yu, Clinton; Wang, Xiaorong; Burke, Anthony M; Chemmama, Ilan E; Kaake, Robyn M; Baker, Peter; Rychnovsky, Scott D; Yang, Jing; Huang, Lan

Wheat, Andrew; Yu, Clinton; Wang, Xiaorong; Burke, Anthony M; Chemmama, Ilan E; Kaake, Robyn M; Baker, Peter; Rychnovsky, Scott D; Yang, Jing; Huang, Lan.

Afiliação

Wheat A; Department of Physiology & Biophysics, University of California, Irvine, CA 92697.
Yu C; Department of Physiology & Biophysics, University of California, Irvine, CA 92697.
Wang X; Department of Physiology & Biophysics, University of California, Irvine, CA 92697.
Burke AM; Department of Chemistry, University of California, Irvine, CA 92697.
Chemmama IE; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94158.
Kaake RM; Gladstone Institutes, University of California, San Francisco, CA 94158.
Baker P; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158.
Rychnovsky SD; Department of Chemistry, University of California, Irvine, CA 92697.
Yang J; State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing 102206, China.
Huang L; Department of Physiology & Biophysics, University of California, Irvine, CA 92697; lanhuang@uci.edu.

Proc Natl Acad Sci U S A ; 118(32)2021 08 10.

Article em En | MEDLINE | ID: mdl-34349018

Assuntos

Reagentes de Ligações Cruzadas/química; Espectrometria de Massas/métodos; Mapeamento de Interação de Proteínas/métodos; Chaperoninas/análise; Chaperoninas/química; Chaperoninas/metabolismo; Química Click/métodos; Células HEK293; Histonas/metabolismo; Humanos; Lisina/química; Complexos Multiproteicos/química; Peptídeos/química; Complexo de Endopeptidases do Proteassoma/metabolismo; Proteômica/métodos; Reprodutibilidade dos Testes; Ubiquitina/metabolismo

Palavras-chave

Alkyne-A-DSBSO; click chemistry enrichment; in vivo cross-linking mass spectrometry; proteinprotein interactions; proteome-wide XL-MS

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Reagentes de Ligações Cruzadas / Mapeamento de Interação de Proteínas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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