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Molecular characterization and antibiotic resistance of Vibrio parahaemolyticus from Indian oyster and their probable implication in food chain.
Parthasarathy, S; Das, Suresh Chandra; Kumar, Ashok; Chowdhury, Goutam; Miyoshi, Shin-Ichi; Dutta, Shanta; Mukhopadhyay, Asish Kumar.
Afiliação
  • Parthasarathy S; Veterinary Public Health Laboratory, ICAR-Indian Veterinary Research Institute (IVRI), Eastern Regional Station, Kolkata, India.
  • Das SC; , Bhubaneswar, Odisha, India.
  • Kumar A; Veterinary Public Health Laboratory, ICAR-Indian Veterinary Research Institute (IVRI), Eastern Regional Station, Kolkata, India.
  • Chowdhury G; Animal Science Division, ICAR, Krishi Bhawan, New Delhi, India.
  • Miyoshi SI; Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases (NICED), P-33, CIT Road, Scheme XM, Beliaghata, Kolkata, 700010, India.
  • Dutta S; Collaborative Research Center of Okayama University for Infectious Diseases at NICED, Kolkata, India.
  • Mukhopadhyay AK; Collaborative Research Center of Okayama University for Infectious Diseases at NICED, Kolkata, India.
World J Microbiol Biotechnol ; 37(8): 145, 2021 Aug 05.
Article em En | MEDLINE | ID: mdl-34351514
Vibrio parahaemolyticus is one of the leading causes of diarrhoea and gastroenteritis in human on consumption of raw or insufficiently cooked seafood. This study was aimed at isolating and characterizing the pathogenic and pandemic V. parahaemolyticus from oysters (n = 90) in coastal parts of West Bengal, India; their antibiotic resistance and potential for involvement in the food chain. During bacteriological culture, typical V. parahaemolyticus colony was recovered in 88.9% samples followed by presumptive identification in 71 (78.9%) samples by characteristic biochemical (K/A) test. All the presumptive isolates (n = 71) were confirmed by species specific Vp-toxR PCR assay. Of these, 10 (14.08%) were tdh+ and none for the trh. Further, 5 (50%) of these tdh+ isolates were found to carry the pandemic potential gene in PGS-PCR assay; however, none in GS-PCR. Majority (80%) of these pathogenic (tdh+) isolates belonged to pandemic serovars (OUT: KUT; OUT: K24; O1: KUT; O1:K25; O10: KUT) and only 20% to non-pandemic serovars (OUT: K15; O9:K17). All the isolates (100%) exhibited resistance to cefpodoxime followed by ampicillin and cefotaxime (90%), ceftizoxime (60%), tetracycline (50%), ceftriaxone (40%), ciprofloxacin and nalidixic acid (10% each). Overall, the study findings suggested that 11.1% (10/90) of commonly marketed oysters in this area were harbouring pathogenic V. parahaemolyticus. Moreover, 5.5% (5/90) of the oyster population were harbouring pandemic strains of this pathogen. Besides, the pathogenic isolates from oysters were exhibiting a considerable genetic relatedness (53 to 70%) to human clinical isolates in PFGE analysis that relates to a substantial public health risk. Further, their multidrug resistance added gravity to the antimicrobial resistance (AMR), a globally growing public health threat and this is a critical area of concern especially during the treatment of foodborne gastroenteritis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ostreidae / Frutos do Mar / Vibrioses / Vibrio parahaemolyticus / Contaminação de Alimentos / Doenças Transmitidas por Alimentos Tipo de estudo: Prognostic_studies Limite: Animals / Humans País como assunto: Asia Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ostreidae / Frutos do Mar / Vibrioses / Vibrio parahaemolyticus / Contaminação de Alimentos / Doenças Transmitidas por Alimentos Tipo de estudo: Prognostic_studies Limite: Animals / Humans País como assunto: Asia Idioma: En Ano de publicação: 2021 Tipo de documento: Article