Rapid interrogation of cancer cell of origin through CRISPR editing.
Proc Natl Acad Sci U S A
; 118(32)2021 08 10.
Article
em En
| MEDLINE
| ID: mdl-34353917
ABSTRACT
The increasing complexity of different cell types revealed by single-cell analysis of tissues presents challenges in efficiently elucidating their functions. Here we show, using prostate as a model tissue, that primary organoids and freshly isolated epithelial cells can be CRISPR edited ex vivo using Cas9-sgRNA (guide RNA) ribotnucleoprotein complex technology, then orthotopically transferred in vivo into immunocompetent or immunodeficient mice to generate cancer models with phenotypes resembling those seen in traditional genetically engineered mouse models. Large intrachromosomal (â¼2 Mb) or multigenic deletions can be engineered efficiently without the need for selection, including in isolated subpopulations to address cell-of-origin questions.
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Base de dados:
MEDLINE
Assunto principal:
Próstata
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Deleção Cromossômica
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Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
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Edição de Genes
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article