A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatin.
Mol Cell
; 81(19): 3979-3991.e4, 2021 10 07.
Article
em En
| MEDLINE
| ID: mdl-34375584
ABSTRACT
Epigenetic inheritance of heterochromatin requires DNA-sequence-independent propagation mechanisms, coupling to RNAi, or input from DNA sequence, but how DNA contributes to inheritance is not understood. Here, we identify a DNA element (termed "maintainer") that is sufficient for epigenetic inheritance of pre-existing histone H3 lysine 9 methylation (H3K9me) and heterochromatin in Schizosaccharomyces pombe but cannot establish de novo gene silencing in wild-type cells. This maintainer is a composite DNA element with binding sites for the Atf1/Pcr1 and Deb1 transcription factors and the origin recognition complex (ORC), located within a 130-bp region, and can be converted to a silencer in cells with lower rates of H3K9me turnover, suggesting that it participates in recruiting the H3K9 methyltransferase Clr4/Suv39h. These results suggest that, in the absence of RNAi, histone H3K9me is only heritable when it can collaborate with maintainer-associated DNA-binding proteins that help recruit the enzyme responsible for its epigenetic deposition.
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Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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DNA Fúngico
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Heterocromatina
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Sequências Reguladoras de Ácido Nucleico
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Metilação de DNA
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Hereditariedade
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Montagem e Desmontagem da Cromatina
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article