FACT is recruited to the +1 nucleosome of transcribed genes and spreads in a Chd1-dependent manner.
Mol Cell
; 81(17): 3542-3559.e11, 2021 09 02.
Article
em En
| MEDLINE
| ID: mdl-34380014
ABSTRACT
The histone chaperone FACT occupies transcribed regions where it plays prominent roles in maintaining chromatin integrity and preserving epigenetic information. How it is targeted to transcribed regions, however, remains unclear. Proposed models include docking on the RNA polymerase II (RNAPII) C-terminal domain (CTD), recruitment by elongation factors, recognition of modified histone tails, and binding partially disassembled nucleosomes. Here, we systematically test these and other scenarios in Saccharomyces cerevisiae and find that FACT binds transcribed chromatin, not RNAPII. Through a combination of high-resolution genome-wide mapping, single-molecule tracking, and mathematical modeling, we propose that FACT recognizes the +1 nucleosome, as it is partially unwrapped by the engaging RNAPII, and spreads to downstream nucleosomes aided by the chromatin remodeler Chd1. Our work clarifies how FACT interacts with genes, suggests a processive mechanism for FACT function, and provides a framework to further dissect the molecular mechanisms of transcription-coupled histone chaperoning.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Proteínas de Grupo de Alta Mobilidade
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Proteínas de Saccharomyces cerevisiae
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Fatores de Elongação da Transcrição
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Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article