Simultaneous Tagmentation-Based Detection of ChIP/ATAC Signal with Bisulfite Sequencing.
Methods Mol Biol
; 2351: 337-352, 2021.
Article
em En
| MEDLINE
| ID: mdl-34382199
ABSTRACT
DNA methylation is thought to regulate accessibility of chromatin and binding of regulatory elements; however, it is difficult to determine if chromatin accessibility or transcription factor (TF) binding overlap with methylated or unmethylated DNA if the assays are performed separately. In order to examine accessibility or TF binding simultaneously with methylation on the same DNA molecule, we developed EpiMethylTag which combines ATAC-Seq or ChIP-Seq (M-ATAC or M-ChIP) with bisulfite conversion. Our approach provides a fast, low-input, low sequencing depth method to determine whether DNAme and accessibility/TF binding are mutually exclusive or can coexist in certain locations.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Cromatina
/
Metilação de DNA
/
Montagem e Desmontagem da Cromatina
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Sequenciamento de Cromatina por Imunoprecipitação
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article