Identification of a Bromodomain-like Region in 15-Lipoxygenase-1 Explains Its Nuclear Localization.
Angew Chem Int Ed Engl
; 60(40): 21875-21883, 2021 09 27.
Article
em En
| MEDLINE
| ID: mdl-34388301
ABSTRACT
Lipoxygenase (LOX) activity provides oxidative lipid metabolites, which are involved in inflammatory disorders and tumorigenesis. Activity-based probes to detect the activity of LOX enzymes in their cellular context provide opportunities to explore LOX biology and LOX inhibition. Here, we developed Labelox B as a potent covalent LOX inhibitor for one-step activity-based labeling of proteins with LOX activity. Labelox B was used to establish an ELISA-based assay for affinity capture and antibody-based detection of specific LOX isoenzymes. Moreover, Labelox B enabled efficient activity-based labeling of endogenous LOXs in living cells. LOX proved to localize in the nucleus, which was rationalized by identification of a functional bromodomain-like consensus motif in 15-LOX-1. This indicates that 15-LOX-1 is not only involved in oxidative lipid metabolism, but also in chromatin binding, which suggests a potential role in chromatin modifications.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Araquidonato 15-Lipoxigenase
/
Inibidores Enzimáticos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article