Imprecise recombinant viruses evolve via a fitness-driven, iterative process of polymerase template-switching events.
PLoS Pathog
; 17(8): e1009676, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-34415977
ABSTRACT
Recombination is a common feature of many positive-strand RNA viruses, playing an important role in virus evolution. However, to date, there is limited understanding of the mechanisms behind the process. Utilising in vitro assays, we have previously shown that the template-switching event of recombination is a random and ubiquitous process that often leads to recombinant viruses with imprecise genomes containing sequence duplications. Subsequently, a process termed resolution, that has yet to be mechanistically studied, removes these duplicated sequences resulting in a virus population of wild type length genomes. Using defined imprecise recombinant viruses together with Oxford Nanopore and Illumina high throughput next generation sequencing technologies we have investigated the process of resolution. We show that genome resolution involves subsequent rounds of template-switching recombination with viral fitness resulting in the survival of a small subset of recombinant genomes. This alters our previously held understanding that recombination and resolution are independent steps of the process, and instead demonstrates that viruses undergo frequent and continuous recombination events over a prolonged period until the fittest viruses, predominantly those with wild type length genomes, dominate the population.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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RNA Polimerase Dependente de RNA
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RNA Viral
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Genoma Viral
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Poliovirus
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Evolução Biológica
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Aptidão Genética
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article