Enhancement of anti-TNF&#945; monoclonal antibody production in CHO cells through the use of UCOE and DHFR elements in vector construction and the optimization of cell culture media.

Doan, Chinh Chung; Ho, Nguyen Quynh Chi; Nguyen, Thi Thuy; Nguyen, Thi Phuong Thao; Do, Dang Giap; Hoang, Nghia Son; Le, Thanh Long

Enhancement of anti-TNFα monoclonal antibody production in CHO cells through the use of UCOE and DHFR elements in vector construction and the optimization of cell culture media.

Doan, Chinh Chung; Ho, Nguyen Quynh Chi; Nguyen, Thi Thuy; Nguyen, Thi Phuong Thao; Do, Dang Giap; Hoang, Nghia Son; Le, Thanh Long.

Afiliação

Doan CC; Department of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Ho NQC; Faculty of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi City, Vietnam.
Nguyen TT; Department of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Nguyen TPT; Department of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Do DG; Department of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Hoang NS; Faculty of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi City, Vietnam.
Le TL; Department of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.

Prep Biochem Biotechnol ; 52(4): 452-470, 2022.

Article em En | MEDLINE | ID: mdl-34427158

Assuntos

Tetra-Hidrofolato Desidrogenase; Fator de Necrose Tumoral alfa; Adalimumab; Animais; Anticorpos Monoclonais/genética; Formação de Anticorpos; Células CHO; Técnicas de Cultura de Células; Cromatina; Cricetinae; Cricetulus; Tetra-Hidrofolato Desidrogenase/genética; Tetra-Hidrofolato Desidrogenase/metabolismo; Fator de Necrose Tumoral alfa/genética

Palavras-chave

CHO-DG44 cell line; DHFR; UCOE element; adalimumab; anti-TNFα monoclonal antibody; gene amplification; medium optimization

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tetra-Hidrofolato Desidrogenase / Fator de Necrose Tumoral alfa Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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