Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas.
Sci Rep
; 11(1): 17100, 2021 08 24.
Article
em En
| MEDLINE
| ID: mdl-34429452
ABSTRACT
To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and characterize them. Primary cell cultures were obtained from two NP tissues (NP2 and NP3) and one SIP tissue (IP4). All the cells were polygonal in shape, expressed cytokeratin 14, and had normal diploid chromosome status. HPV58 DNA was detected in NP3. To obtain immortal primary cells, NP2 and IP4 cells were transduced with a combination of mutant CDK4, cyclinD1 and TERT. These cells were thereafter named NP2/K4DT and IP4/K4DT, respectively. HPV58-positive NP3 cells were transduced with TERT alone, the resulting cells named NP3/T. Phenotypic and genotypic identity of original tissues and derived cells was investigated. All the cell cultures with transgenes were confirmed to be derived from their parental cells and primary tumor tissues by analysis of short tandem repeats (STR) and maintained in vitro growth, genetic profiles and gene expression characteristics of the primary cells. These virtually immortalized cells, as well as the primary cells, have potential as in vitro models for studying the pathogenesis of NPs and SIPs and for preclinical study to develop new therapeutic agents.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pólipos Nasais
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Neoplasias Nasais
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Papiloma Invertido
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Técnicas de Cultura de Células
Tipo de estudo:
Prognostic_studies
Limite:
Adolescent
/
Aged
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Child
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Humans
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Male
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article