A miniaturized optoelectronic biosensor for real-time point-of-care total protein analysis.
MethodsX
; 8: 101414, 2021.
Article
em En
| MEDLINE
| ID: mdl-34430309
A miniaturized optoelectronic sensor is demonstrated that measures total protein concentration in serum and urine with sensitivity and accuracy comparable to gold-standard methods. The sensor is comprised of a vertical cavity surface emitting laser (VCSEL), photodetector and other custom optical components and electronics that can be hybrid packaged into a portable, handheld form factor. In conjunction, a custom fluorescence assay has been developed based on the protein-induced fluorescence enhancement (PIFE) phenomenon, enabling real-time sensor response to changes in protein concentration. Methods are described for the following:â¢Standard curves: Used to determine the sensitivity, dynamic range, and linearity of the VCSEL biosensor/PIFE assay system in buffer as well as in human blood and urine samples.â¢Comparison of VCSEL biosensor performance with a benchtop fluorimetric microplate reader.â¢Accuracy of the VCSEL biosensor/PIFE assay system: Evaluated by comparing sensor measurements with gold-standard clinical laboratory measurements of total protein in serum and urine samples from patients with diabetes.
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MEDLINE
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En
Ano de publicação:
2021
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Article