Axonal Injuries Cast Long Shadows: Long Term Glial Activation in Injured and Contralateral Retinas after Unilateral Axotomy.
Int J Mol Sci
; 22(16)2021 Aug 07.
Article
em En
| MEDLINE
| ID: mdl-34445225
ABSTRACT
BACKGROUND:
To analyze the course of microglial and macroglial activation in injured and contralateral retinas after unilateral optic nerve crush (ONC).METHODS:
The left optic nerve of adult pigmented C57Bl/6 female mice was intraorbitally crushed and injured, and contralateral retinas were analyzed from 1 to 45 days post-lesion (dpl) in cross-sections and flat mounts. As controls, intact retinas were studied. Iba1+ microglial cells (MCs), activated phagocytic CD68+MCs and M2 CD206+MCs were quantified. Macroglial cell changes were analyzed by GFAP and vimentin signal intensity.RESULTS:
After ONC, MC density increased significantly from 5 to 21 dpl in the inner layers of injured retinas, remaining within intact values in the contralateral ones. However, in both retinas there was a significant and long-lasting increase of CD68+MCs. Constitutive CD206+MCs were rare and mostly found in the ciliary body and around the optic-nerve head. While in the injured retinas their number increased in the retina and ciliary body, in the contralateral retinas decreased. Astrocytes and Müller cells transiently hypertrophied in the injured retinas and to a lesser extent in the contralateral ones.CONCLUSIONS:
Unilateral ONC triggers a bilateral and persistent activation of MCs and an opposed response of M2 MCs between both retinas. Macroglial hypertrophy is transient.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Células Ganglionares da Retina
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Axônios
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Microglia
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Axotomia
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Traumatismos do Nervo Óptico
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article