A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX.
Int J Mol Sci
; 22(16)2021 Aug 19.
Article
em En
| MEDLINE
| ID: mdl-34445629
ABSTRACT
General cancer-targeted ligands that can deliver drugs to cells have been given considerable attention. In this paper, a high-affinity DNA aptamer (HG1) generally binding to human tumor cells was evolved by cell-SELEX, and was further optimized to have 35 deoxynucleotides (HG1-9). Aptamer HG1-9 could be taken up by live cells, and its target protein on a cell was identified to be human transferrin receptor (TfR). As a man-made ligand of TfR, aptamer HG1-9 was demonstrated to bind at the same site of human TfR as transferrin with comparable binding affinity, and was proved to cross the epithelium barrier through transferrin receptor-mediated transcytosis. These results suggest that aptamer HG1-9 holds potential as a promising ligand to develop general cancer-targeted diagnostics and therapeutics.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Receptores da Transferrina
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Aptâmeros de Nucleotídeos
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Técnica de Seleção de Aptâmeros
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Neoplasias
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article