Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit.
Viruses
; 13(8)2021 07 29.
Article
em En
| MEDLINE
| ID: mdl-34452352
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2'-O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2'-O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Capuzes de RNA
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RNA Viral
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Proteínas não Estruturais Virais
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Proteínas Virais Reguladoras e Acessórias
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SARS-CoV-2
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COVID-19
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Metiltransferases
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article